Experience in laboratory experiments in molecular biology is important to students, researchers and practioners in the biolo- gical and medical sciences. This book is designed to introduce the reader to basic methods used in the isolation, cloning and analysis of genetic material. The text includes theory and fundamental methods in suffi- cient experimental detail which have been successfully used in our studies of viral genome and eukaryotic gene regulation. Spe- cific protocols together with troubleshooting tips are given for RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagene- sis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analy- sis. The methods chosen have been approved in several interna- tional Molecular Biology Courses and without doubt can be used as a manual in basic molecular studies of DNA and RNA. The editor is grateful to all the contributors for their coopera- tion and would like to express his thanks to the publishers, espe- cially Dr. J. Lindenborn from Springer-Verlag, for their coopera- tion, patience and assistance during the preparation of this book. Riga, Latvia V. BERZINS Contents Chapter 1 cDNA Synthesis and Cloning ...1 JURIS STEINBERGS AND ALEKSANDER TSIMANIS Chapter 2 DNA Sequencing...22 ...ERIKS JANKEVICS Chapter 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase...43 ...ELITA AVOTA AND NORMUNDS LICIS Chapter 4 PCR-Based Site-Specific Mutagenesis...53 ...JANIS KLOVINS AND VALDIS BERZINS Chapter 5 Analysis of Specific Protein-DNA Interactions...68 . .
The second original, touching, twisted, and most of all hilarious novel and audiobook for children performed by David Walliams and Matt Lucas. This wonderful story is told by two of Britains best loved comedians and the characters from David's book spring to life with fantastic voices and characters galore that will make you laugh out loud. READ BY DAVID WALLIAMS and MATT LUCAS "Mr Stink stank. He also stunk. And if it was correct English to say he stinked, then he stinked as well..." Chloe sees Mr Stink every day, but she's never spoken to him. Which isn't surprising, because he's a tramp, and he stinks. But there's more to Mr Stink than The Decipherment of Linear B download ebook pdf meets the eye (or nose) and before she knows it, Chloe has an unusual new friend hiding in her garden shed. As Chloe struggles to keep Mr Stink a secret, and her dad tries to hide a secret of his own, the stage is set for an epic family confrontation. But there's one other person with an extraordinary secret - Mr Stink himself...
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Author: Valdis Berzins
Number of Pages: 169 pages
Published Date: 07 Jul 2012
Publisher: Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Publication Country: Berlin, Germany
Language: English
ISBN: 9783642489778
Download Link: Click Here
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